Optimization of plant regeneration system in vitro culture in wheat

Qin Jianbing, Wang Yuesheng, and He Guangyuan

Abstract: Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results howed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency han Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as ollowing: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root ifferentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per allus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.

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