Molecular characterization of low molecular weight glutenin genes from Yunnan hulled wheat (Triticum aestivum subsp yunnanense King)

J.Y. Fang, Z.J. Li, G.X. Yang and G.Y. He

Abstract：Three new low molecular weight glutenin subunits (LMW-GS) genes, designated LMWYN-1, LMWYN-2 and LMWYN-3, were isolated and characterized from Yunnan hulled wheat accession YN11 (Triticum aestivum subsp. yunnanense King). The length of complete nucleotide sequence of LMWYN-1, LMWYN-2 and LMWYN-3 were 1056, 960 and 897 bp, respectively. LMWYN-1 and LMWYN-2 encoded proteins with 330 and 299 amino acid residues, respectively, whereas LMWYN-3 was a putative pseudogene due to the presence of three stop codons in the coding region. All deduced amino acid sequences of the three genes were the LMW-m type according to a methionine at position 1 of the putative mature protein. In repetitive domain of LMWYN-1 two hydrophobic motifs PIIIL and PVIIL were observed. LMWYN-1, LMWYN-2 and LMWYN-3 were classified as groups 5, 2 and 8, respectively. Phylogenetic analysis showed that LMWYN-1 and LMWYN-3 were closely related to the genes on chromosome 1D and LMWYN-2 was homologous with the genes on chromosome 1B.

Link: http://scialert.net/abstract/?doi=ajps.2008.461.466

Doi: 10.3923/ajps.2008.461.466