Effect of the atmospheric pressure nonequilibrium plasmas on the conformational changes of plasmid DNA

Xu Yan,Fei Zou,Xin Pei Lu, Guangyuan He,Meng Jun Shi,Qing Xiong,Xuan Gao,Zilan Xiong,Yin Li,Feng Yun Ma,Men Yu,Chang Dong Wang,Yuesheng Wang,and Guangxiao Yang

Abstract:The cold atmospheric pressure plasma, which has been widely used for biomedical applications,may potentially affect the conformation of DNA. In this letter, an atmospheric pressure plasma plume is used to investigate its effects on the conformational changes of DNA of plasmid pAHC25.It is found that the plasma plume could cause plasmid DNA topology alteration, resulting in the percentage of the supercoiled plasmid DNA form decreased while that of the open circular and linearized form of plasmid DNA increased as detected by agrose gel electrophoresis. On the other hand, further investigation by using polymerase chain reaction method shows that the atmospheric pressure plasma jet treatments under proper conditions does not affect the genes of the plasmid DNA, which may have potential application in increasing the transformation frequency by genetic engineering.

Link:

http://aip.scitation.org/doi/10.1063/1.3212739

DOI: 10.1063/1.3212739