Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes

Peng Chen, Ru Li ,Ruiyang ,ZhouZhiguo, Guangyuan He

Abstract

Four novel low molecular weight glutenin subunit genes (designated as AmLMWm1, AcLMWm1, AcLMWm2 and AcLMWm3) were isolated and characterized from the genomic DNA of two Aegilops accessions, AmLMWm1 from Aegilops markgrafii (Greuter) K.Hammer (2n = 2x = 14, CC), AcLMWm1, AcLMWm2 and AcLMWm3 from Aegilops cylindrica Host (2n = 4x = 14, CCDD). The coding regions of AmLMWm1, AcLMWm1, AcLMWm2 and AcLMWm3 were 1,092, 1,119, 912 and 936 bp in length, encoding 39,168, 40,297, 31,961 and 33,396 Da LMW-GS, respectively. In order to confirm their authenticity, these LMW-GS genes were successfully expressed in E.coli. The four novel genes belong to LMW-m types and, according to the first and seventh cysteine positions, were further classified as types III, I, IV and V, respectively. Among these novel genes, AmLMWm1 and AcLMWm1 possessed a longer repetitive domain (20 and 22 repeats) than common LMW-GS genes, which was considered to be associated with good dough quality of wheat. Phylogenetic analysis showed C and D genomes of Aegilops involved in this study had LMW-GS loci similar to those of the A, B, and D genomes in common wheat.

Link:

https://link.springer.com/article/10.1007/s10722-009-9527-y

DOI: 10.1007/s10722-009-9527-y