Overexpression of soybean GmCBL1 enhances abiotic stress tolerance and promotes hypocotyl elongation in Arabidopsis

Li, ZY (Li, Zhi-Yong) ; Xu, ZS (Xu, Zhao-Shi) ; He, GY (He, Guang-Yuan); Yang, GX (Yang, Guang-Xiao) ; Chen, M (Chen, Ming) ; Li, LC (Li, Lian-Cheng) ; Ma, YZ (Ma, You-Zhi)

Abstract:Although extensive studies and remarkable progress have been made with Arabidopsis calcineurin B-like proteins (CBLs), knowledge of their functions in other plant species is still limited. Here we isolated gene GmCBL1 from soybean, a homolog of AtCBL1 in Arabidopsis. GmCBL1 was differentially induced by multiple abiotic stress and plant hormones, and its transcripts were abundant in seedlings and mature roots. We over-expressed GmCBL1 in Arabidopsis and found that it enhanced tolerances to both high salt and drought stresses in the transgenic plants. Overexpression of GmCBL1 also promoted hypocoty l elongation under light conditions. GmCBL1 may regulate stress tolerance through activation of stress-related genes, and may control hypocotyl development by altering the expression of gibberellin biosynthesis-related genes. This study identifies a putative soybean CBL gene that functions in both stress tolerance and light-dependent hypocotyl development.

Link:https://ac.els-cdn.com/S0006291X12019067/1-s2.0-S0006291X12019067-main.pdf?_tid=a212d496-c09a-11e7-a0a6-00000aab0f6c&acdnat=1509715783_42668a4d4731779edb2d36af03a63a71

DOI: 10.1016/j.bbrc.2012.09.128