Agrobacterium- mediated tobacco transformation with wheat DREB2 gene.

M Rashid ， GY He ， GX Yang

Abstract: Plants encounter various biotic and abiotic stresses at different growth stages throughout their life. To grapple and adjust with persisting stress conditions, plants have developed certain mechanisms through the mean of transcription. Transcription factors manipulate the feat of multiple stress response in a contemporized way. Among them, drought resistant element binding proteins DREB may act as a defense impediment to minimize plant water loss. The DREB2 gene (PTTa00075.1）amplified from wheat cv. Chinese spring was cloned in pCAMBIA 1304 under the control of CaMV35S promoter. To modify the genome and plant traits, Agrobacterium-mediated transformation may transmit the desired genes. The transgenic tobacco plants harboring the desired gene were developed through tissue culture technique. The GUS (β-glucuronidase) staining confirms the gene transformation. The putative transgenic plants were selected on selective antibiotic (hygromycin) media. The PCR analysis of T1 generation confirmed the incorporation of the transgene into the tobacco genome for further evaluation.

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Link: https://www.researchgate.net/publication/269986187_Agrobacterium-_mediated_tobacco_transformation_with_wheat_DREB2_gene