Wheat CBL-interacting protein kinase 25 negatively regulates salt tolerance in transgenic wheat

X Jin ， T Sun ， X Wang ， P Su ， J Ma ， ...

Abstract
CBL-interacting protein kinases are involved in plant responses to abiotic stresses, including salt stress. However, the negative regulating mechanism of this gene family in response to salinity is less reported. In this study, we evaluated the role of TaCIPK25 in regulating salt response in wheat. Under conditions of high salinity,TaCIPK25expression was markedly down-regulated in roots. Overexpression of TaCIPK25resulted in hypersensitivity to Na+and superfluous accumulation of Na+in transgenic wheat lines.TaCIPK25expression did not decline in transgenic wheat and remained at an even higher level than that in wild-type wheat controls under high-salinity treatment. Furthermore, transmembrane Na+/H+exchange was impaired in the root cells of transgenic wheat. These results suggested that TaCIPK25 negatively regulated salt response in wheat. Additionally, yeast-one-hybrid, -glucuronidase activity and DNA-protein-interaction-enzyme-linked-immunosorbent assays showed that the transcription factor TaWRKY9 bound W-box in theTaCIPK25promoter region. Quantitative real-time polymerase chain reaction assays showed concomitantly inverted expression patterns ofTaCIPK25andTaWRKY9in wheat roots under salt treatment, ABA application and inhibition of endogenous ABA condition. Overall, based on our results, in a salt stress condition, the negative salt response in wheat involved TaCIPK25 with the expression regulated by TaWRKY9.

Link:http://xueshu.baidu.com/s?wd=Wheat+CBL-interacting+protein+kinase+25+negatively+regulates+salt+tolerance+in+transgenic+wheat&tn=SE_baiduxueshu_c1gjeupa&cl=3&ie=utf-8&bs=Identification+of+the+ASR+gene+family+from+Brachypodium+distachyon+and+functional+chara

DOI: 10.1038/srep28884